人*磷酸甘油酯(PC/CPG)檢測試劑盒Human choline phosphoglyceride,PC/CPG ELISA試劑盒齊一生物科技（上海）有限公司以真誠、主動、周到、規范、熱情的服務很快得到廣大客戶的*好評.公司秉承“適應多樣化的需求,提供專業化的服務"的經營理念,為客戶提供*的服務

人*磷酸甘油酯(PC/CPG)檢測試劑盒ELISA試劑盒操作步驟
ELISA試劑盒使用前,將所有試劑充分混勻.不要使液體產生大量的泡沫,以免加樣時加入大量的氣泡,產生加樣上的誤差.
根據待測樣品數量加上標準品的數量決定所需的板條數.每個標準品和空白孔建議做復孔.每個樣品根據自己的數量來定,能使用復孔的盡量做復孔.標本用標本稀釋液1：1稀釋后加入50ul于反應孔內.
加入稀釋好后的標準品50ul于反應孔、加入待測樣品50ul于反應孔內.立即加入50ul的*標記的抗體.蓋上膜板,輕輕振蕩混勻,37℃溫育1小時.
甩去孔內液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復此操作3次.如果用洗板機洗滌,洗滌次數增加一次.
每孔加入80ul的親和鏈酶素-HRP,輕輕振蕩混勻,37℃溫育30分鐘.
甩去孔內液體,每孔加滿洗滌液,振蕩30秒,甩去洗滌液,用吸水紙拍干.重復此操作3次.如果用洗板機洗滌,洗滌次數增加一次.
每孔加入底物A、B各50ul,輕輕振蕩混勻,37℃溫育10分鐘.避免光照.
取出酶標板,迅速加入50ul終止液,加入終止液后應立即測定結果.
在450nm波長處測定各孔的OD值.
 20X PBS Tween-20 Buffer      500 mL 
 20X TAE Buffer      500 mL 
 10X TBE Buffer      1 L 
 20X TBS Buffer      500 mL 
 20X TBS Tween-20 Buffer      0.5 L 
 10X Tris-Glycine SDS Buffer      1000 mL 
 10X Tris-Glycine Buffer      1 L 
 Sodium Dodecyl Sulfate (SDS), Lauryl      100 GM 
 Sodium Dodecyl Sulfate (SDS), Lauryl      1000 GM 
 20X Tris-HEPES-SDS Buffer      500 mL 
 BupH Phosphate Buffered Saline Packs      40 PACKS/PKG 
 BupH Modified Dulbecco's PBS Packs      40 PACKS/PKG 
 BupH Tris Buffered Saline Packs      40 PACKS/PKG 
 BupH Tris-Glycine-SDS Buffer Packs      40 PACKS/PKG 
 BupH Tris Buffered Saline Packs      10 PACK 
 BupH Tris-Glycine Buffer Packs      40 PACKS/PKG 
人*磷酸甘油酯(PC/CPG)檢測試劑盒 BupH Carbonate-Bicarbonate Buffer Packs      40 PACKS/PKG 
 BupH Borate Buffer Packs      40 PACKS/PKG 
 BupH MES Buffered Saline Packs      10 PACKS 
 BupH Tris-HEPES-SDS Running Buffer      10 PACK 
 Pierce Iodination Reagent      1 g 
 Pierce Iodination Tubes      10 TUBES/PKG 
 Pierce Iodination Beads      50/PKG 
 Pierce Iodination Beads      250/PKG 
 Trifluoroacetic Acid (TFA), Sequencing grade      500 mL 
 Trifluoroacetic Acid (TFA), Sequencing grade      10/1 GM 
 Trifluoroacetic Acid (TFA), Sequencing grade      100 GM 
 Trifluoroacetic Acid (TFA), Sequencing grade      10 X 1 mL 
 Formic Acid, LC-MS Grade      10 X 1 mL 
 16% Formaldehyde (w/v), Methanol-free      10 X 1 mL 
 16% Formaldehyde (w/v), Methanol-free      10 X 10 mL 
 TNBSA Solution      100 mL 
 Biotin      1 g 
 Horseradish Peroxidase, Biotinylated      5 mg 
 NeutrAvidin Agarose      5 mL 
 NeutrAvidin Agarose      10 mL 
 High Capacity NeutrAvidin Agarose      5 mL 
 High Capacity NeutrAvidin Agarose      10 mL 
 Alkaline Phosphatase, Biotinylated      1 mg 
 Vacuum Hydrolysis Tube, 1mL      1 Tube 
 Vacuum Hydrolysis Tube, 6mL      1 Tube 
 Vacuum Hydrolysis Tube, 18mL      1 Tube 
 Urea, Sequanal Grade      1000 GM 
 Ethylene Glycol      200 mL 
Human TGF beta 1
Human TGF-beta-3
Human THPO
Human TIMP-1
Human Tissue Factor
Human TNF-alpha
Human TNF-alpha
Human TNF-beta
Human TNF-R1
Human TNF-R2
Human TREM-1
Human VCAM-1
Human VCAM-1 [109-331]
Human VEGF-121
Human VEGF-165
Human VEGF-165
Human VEGFR-1
Human Visfatin
Mouse aFGF
Mouse bFGF
Mouse CD40L
Mouse EBI-3
Mouse EGF
Mouse FGF-9
Mouse FLT-3 Ligand
Mouse G-CSF
Mouse GM-CSF
Mouse IFN-gamma
Mouse IGF-1
Mouse IL-15
Mouse IL-16
Mouse IL-17-A
Mouse IL-2
Mouse IL-27/p28
Mouse IL-3
Mouse IL-4
Mouse IL-6
Mouse IL-7
Mouse IL-9
Mouse IP-10
Mouse Leptin
Mouse MCP-3
Mouse MCSF
Mouse MIP-1-alpha
Mouse MIP-1-beta
人*磷酸甘油酯(PC/CPG)檢測試劑盒Mouse MIP-3-alpha
Mouse MIP-3-beta
Mouse NGF-beta
Mouse PDGF-AA
Mouse PDGF-BB
Mouse Rank Ligand
Mouse Rantes
Mouse Relm alpha
Mouse Relm beta
Mouse SCF
Mouse SDF-1-alpha
Mouse Sonic Hedgehog
Mouse THPO
Mouse TNF-alpha
Mouse VEGF-120
Mouse VEGF-165
Rat FGF-9
Rat IFN-gamma
Rat IGF-1