詳細介紹
免疫球蛋白M(IgM)檢測試劑盒(血清.血漿.組織)Human Immunoglobulin M,IgM ELISA Kit 齊一生物科技(上海)有限公司以真誠、主動、周到、規范、熱情的服務很快得到廣大客戶的*好評.公司秉承“適應多樣化的需求,提供專業化的服務"的經營理念,為客戶提供*的服務
免疫球蛋白M(IgM)檢測試劑盒(血清.血漿.組織)類別:酶聯免疫分析檢測試劑盒
規格:96T/48T
包裝:盒
檢測原理:采用雙抗夾心法
保質期:六個月
產品保存條件:2-8°C
應用范圍:僅供科研研究實驗使用
樣品形式:血清/血漿/組織勻漿/細胞培養上清//其它生物液體(具體情況請咨詢)
標本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存 過程 中 如有沉淀形成,應再次離心。
2.血漿:應根據標本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。胸腹水、腦脊液參照此實行。
4.細胞培養上清:檢測分泌性的成份時,用無菌管收集。離心20分鐘左右(2000-3000轉/ 分)。仔細收集上清。 檢測細胞內的成份時,用PBS(PH7.2-7.4)稀釋細胞懸液,細胞濃度達到100萬/ml左右。通過反復凍融,以 使細胞破壞并 放出細胞內成份。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
5.組織標本:切割標本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存備用。標本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標本勻漿充分。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。分裝后一份待檢測,其余冷凍備用。
標本要求:
1.標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
Instrument Part 9239156 Assembly, gripper, TWI
Instrument Part 9239363 Inlet-micro-fuse T 3.15A, 250V, EZ1
Consumable 132519 NeXtal Stock Ammonium sulfate (50) 3.5M Ammonium sulfate - 50mL (4 x 12,5 ml tube)
Consumable 132554 NeXtal Stock Imidazole HCl (50) 1M Imidazole HCl - 50mL (4 x 12,5 ml tube)
Consumable 132629 NeXtal Stock Sodium citrate untit. (50) 1M tri-Sodium citrate dihydrate - Untitrated - 50mL (4 x 12,5 ml tube)
Instrument Part 9019578 Cable, Earthlink 85 mm, Cxx
Instrument Part 9019591 O-ring, sink separator, CX1
Instrument Part 9019593 Belt, Z-axis gearbox (grey), 8 ch., Cx8
Instrument Part 9019622 Cable, P assembly, 1ch., Cx2
Consumable 9019063 PyroMark Q24 MDx Software For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
Consumable 979105 PyroMark Q96 HS Tip Storage Box
Consumable 979202 PyroMark Q24 Cartridge (3) For delivery of nucleotides and reagents for use in PyroMark Q24.
Instrument Part 9019459 Transformer (115V), HS
Instrument Part 981103 Strip Tubes and Caps, 0.1 ml (250)
Consumable 335452 EpiTect? Methyl II DNA Restriction Kit
Instrument Part 9017840 Tool, calibration tip, QIAC
Consumable 69504 DNeasy Blood & Tissue Kit (50) 50 DNeasy Spin Columns, QIAGEN Proteinase K, Buffers, Collection Tubes (2 ml)
Consumable 74126 RNeasy Protect Mini Kit (250) RNAlater RNA Stabilization Reagent (250 ml), 250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
Consumable 201225 Taq PCR Core Kit (1000) "4 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2, dNTP Mix†
NEW! Now with 10x CoralLoad PCR Buffer
Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."
Consumable 301427 Effectene Transfection Reagent (4x1ml) 4 x 1 ml Effectene Reagent, Enhancer, Buffer; for 160 transfections in 60 mm dishes or 640 transfections in 12-well plates
Consumable 962141 QIAprep 96 Turbo BioRobot Kit (4) For 4 x 96 high-purity plasmid minipreps, 4 each: TurboFilter 96 and QIAprep 96 Plates; Flat-Bottom Blocks and Lids, Reagents, Buffers, Collection Microtubes (1.2 ml) and Caps, 96-Well Microplates RB and Lids, Tape Pads
Consumable 132510 NeXtal Stock ADA untit. (50) 0.5M ADA - Untitrated - 50mL (4 x 12,5 ml tube)
Consumable 132528 NeXtal Stock CAPSO untit. (50) 0.5M CAPSO - Untitrated - 50mL (4 x 12,5 ml tube)
Consumable 132578 NeXtal Stock PEG 2,000 DME (50) 50% w/v Polyethylene glycol 2,000 dimethyl ether - 50mL (4 x 12,5 ml tube)
Consumable 132635 NeXtal Stock Zinc chloride (50) 0,1M Zinc chloride - 50mL (4 x 12,5 ml tube)
Instrument Part 9019572 Pulley, Idler X-axis, Cxx
Instrument Part 9019600 O-ring, filter housing seal, 2mm, CX1
Instrument Part 9019615 Pipettor, spring, Cx2
Instrument Part 9019618 Motor, Z-axis, Cx2
9019663 PyroMark Vac Prep Tool, PyroQ96VW
Consumable 929706 QX 0.2 ml 12-Tube Strip Caps (80)
Consumable 972442 PyroMark Q96 SNP HFE (96) For detection of variants in HFE gene
Consumable 972807 PyroMark Gold Q96 Reagents (50 x 96) For performing pyrosequncing reaction on the Q96 MD
Consumable 979013 Fungi ITS2 Primer 1 for Sequencing
Instrument Part 9019453 Front label incl. cutout for P/R, HS
Consumable 990604 Elution Plate 8-Strip Caps (120)
Instrument Part 981106 Strip Tubes and Caps, 0.1 ml (2500)
Instrument Part 981604 Rotor-Disc Heat Sealing Film (600)
Consumable 950207 Reagent Pack, VX The Reagent Pack, VX, which is used together with the Virus Plasticware Pack, QIAxtractor (#950047) provides fully automated viral nucleic acid purification on the QIAxtractor. The combination of reagents and plasticware enables purification of viral nucleic acids from liquid samples, with a throughput of 8-96 samples per run.
Consumable 288115 bactotype Mg/Ms PCR Reagent (96)
Instrument Part 9022655 Riser Block EMTR, QXT
Consumable 10091 QIAGEN-tip 10000 (5) 5 columns; 10 mg column capacity
Consumable 51304 QIAamp DNA Mini Kit (50) For 50 DNA preps: 50 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)
Consumable 201203 Taq DNA Polymerase (250) "250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2
NEW! Now with 10x CoralLoad PCR Buffer
Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."
Accessory 19571 AirPore Tape Sheets (50) Microporous tape sheets for covering 96-well blocks: 50 sheets per pack
Consumable 19763 QIAfilter Maxi Cartridges (25) 25 QIAfilter Maxi Cartridges
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