詳細介紹
前列腺分泌蛋白94(PSP94)檢測試劑盒英文名稱Human Prostate secretory protein94,PSP94 ELISA kit齊一生物*經營ELISA試劑盒(免費代測)及抗體,分子學生物試劑盒,金標法檢測試劑盒,比色法檢測試劑盒,細胞菌株,培養基,細胞因子,生化試劑,耗材等生物試劑產品.誠信經營,價格實惠,服務周到,質量保障!提供各種實驗代測服務.歡迎新老客戶
前列腺分泌蛋白94(PSP94)檢測試劑盒類別:酶聯免疫分析檢測試劑盒
規格:96T/48T
包裝:盒
檢測原理:采用雙抗夾心法
保質期:六個月
產品保存條件:2-8°C
應用范圍:僅供科研研究實驗使用
樣品形式:血清/血漿/組織勻漿/細胞培養上清//其它生物液體(具體情況請咨詢)
標本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存 過程 中 如有沉淀形成,應再次離心。
2.血漿:應根據標本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。胸腹水、腦脊液參照此實行。
4.細胞培養上清:檢測分泌性的成份時,用無菌管收集。離心20分鐘左右(2000-3000轉/ 分)。仔細收集上清。 檢測細胞內的成份時,用PBS(PH7.2-7.4)稀釋細胞懸液,細胞濃度達到100萬/ml左右。通過反復凍融,以 使細胞破壞并 放出細胞內成份。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
5.組織標本:切割標本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存備用。標本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標本勻漿充分。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。分裝后一份待檢測,其余冷凍備用。
標本要求:
1.標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
9019637 Cable, UV lamp volt. conv., long, EZ1xl
9019590 Cover, plexiglas, 8 ch., CX1
9019565 Clamp, cable 2 wires 2 tabs, Cxx
9019543 Bow spring, table rail, Cxx
9019510 CHAMBER MAIN VAC STATION 1820
9019505 PLATE SEPARATOR 48 WELL HIGH
204256 QuantiFast Probe PCR Kit (2000)
204174 Rotor-Gene SYBR Green RT-PCR Kit (400) The Rotor-Gene SYBR Green RT-PCR Kit is dedicated for use with the Rotor-Gene Q cycler, providing ultrafast, highly specific quantification in real-time one-step RT-PCR using SYBR Green I.
204154 QuantiFast SYBR Green RT-PCR Kit (400)
9019424 Wiring, lower heater, RG3
9019417 Fan, lid, RG3
9019393 Solenoid, cooling with flap, RG3
9019386 Photo Multiplier Tube with cable, RG3
9019368 Damper, lid, RG6
9019347 Motor, 12VDC Heater fan, RG6
9234697 Manual Hardware Plato 7
9020477 Gasket, front plate, GSC
9234463 Coupling, CPC, inline, female, 8mm
1027285 AllStars Neg. siRNA AF 546 (5 nmol)
1022564 Mm/Hs_MAPK1 control siRNA (5 nmol)
9019205 Opto - Stacker, PyroQ96MD
9019204 Opto - Robot Z motor , PyroQ96MD
9019200 Hinge kit - Top cover, PyroQ96MD
1022073 Luciferase GL3 siRNA (5 nm)
1022064 GFP-22 siRNA (5 nm)
1018996 PCR Buffer, 10 x (22ml/30),KG
1064605 MagAttract M48 DNA Manual Kit (200)
1066851 PG FMDV RT-PCR Assay (48) LE Z
1061054 HBV PCR REAGENT I
1061069 UU PCR REAGENT II
1061079 NG PCR REAGENT II
9019886 Lid, label 4CH, RG3
9019875 Cover, main, silver, RGQ
9019836 IPC Hard Drive PCA6751, PyroQ96ID
9019769 Valve, shutoff, V2, QX
9019756 Valve, solenoid, V2, QX
9019734 Sensor, Z, with cable, V2, QX
9019728 Board, motor control, Y, V2, QX
9013832 Reagent Holder, 1-bottle Holder for accommodating 1 bottle (5 ml). For use with QIAGEN Protease on the BioRobot MDx worktable
9013824 BGR Tischplatte P3041 Robotec(SN602)
9013719 RS 232 Servicecabel
9013506 96-well Thermostat Base Plate1.02
9013484 Cooling and heating system, BR3000
9013442 Centrifuge, strip adaptor set
9013434 Maintenance kit, annual, BR9600 (A)
9019723 Transport lock, EZ1xl
9019656 Hose w. quick lock socket, PyroQ96VW
9019581 Pulley, Idler Y-axis, Cxx
9019554 Rail, Z-axis 138 mm, Cxx
9019552 Rail, X-axis 444 mm, CQx
218060 miScript Reverse Transcription Kit (10)
216513 FastLane Cell Multiplex Kit (200)
204254 QuantiFast Probe PCR Kit (400)
9019440 Solid State Relay
9019433 Transformer 2x115VAC O/P 2x11VAC
9019431 Kit, lock ring 36W incl. lockknob, RG3
9019402 Transformer, heater, RG3
1027321 Mm/Hs_MAPK1 control siRNA (20 nmol)
9019329 Serial connector USB, internal cable, RG
9019238 Heating block, PyroQ24
9020478 Heating plate, GSC
1026965 PCR Buffer,10 x (1,2/2) without MgCl2
Instrument Part 9018145 Adapter, heated shaker, Qsym
Instrument Part 9018173 PCB, connector, portal, Qsym
Instrument Part 9240946 Encoder, y, portal, Qsym
Instrument Part 9240956 Cable, handler, Qsym
Instrument Part 9240979 Cable, sample tracking, Qsym
Replacement Component 1067930 Buffer ALO (10 ml)
Replacement Component 1068797 Buffer DLB (13mg lyophylized), G
Consumable 387016 Investigator Quantiplex Kit (200)
Consumable 288315 cador Salmonella PCR Reagent (96)
Consumable 979102 PyroMark Q96 HS Q96 Reagent Tip (4) Re-usable tips for dispensing reagents (RDTs) for use in PyroMark Q96 MD. Each package contains 4 tips.
Instrument Part 990550 Tip Disposal Box (10)
Instrument Part 990556 Reagent Trough Lid 170 mL (20)
Consumable 950921 Capture Plate, VX (5)
337012 FDK design fee
4838024 careNG PCR ASSAY V2
Consumable 870601 T315I RGQ PCR Kit (24)
Instrument Part 9022656 Channeling Adapter, QXT
Consumable 10023 QIAGEN-tip 20 (25) 25 columns; 20 μg column capacity
Consumable 19056 Buffer QF 1000 ml Elution Buffer
Consumable 19054 Buffer QBT 1000 ml Equilibration Buffer
Consumable 30210 Ni-NTA Agarose (25 ml) 25 ml nickel-charged resin (max. pressure: 2.8 psi)
Consumable 51306 QIAamp DNA Mini Kit (250) For 250 DNA preps: 250 QIAamp Mini Spin Columns, QIAGEN Proteinase K, Reagents, Buffers, Collection Tubes (2 ml)
Consumable 19077 Buffer AE 240 ml Elution Buffer for 1000 preps
Accessory 19131 QIAGEN Proteinase K (2) 2 ml (>600 mAU/ml, solution)
Accessory 19566 Collection Microtube Caps Nonsterile polypropylene caps for collection microtubes (1.2 ml) and round-well blocks, 960 in strips of 8
Accessory 19579 Flat-Bottom Blocks (24) 96-well blocks with 2 ml wells: 24 per case, lids included
Consumable 19585 S-Blocks (24) "Succsessor of
#19573 Square-Well Blocks (24)"
Consumable 28006 MinElute PCR Purification Kit (250) 250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
Consumable 28183 QIAquick 96 PCR Purification Kit (24) For purification of 24 x 96 PCR reactions: 24 QIAquick 96 Plates, Buffers, Collection Microtubes (1.2 ml), Caps
9017849 Swing-out Buckets (2)
Instrument Part 9240630 Power Supply, 5VDC 1.5 AMP, AP96
Instrument Part 9240663 Nut, Flangeless,1/4-28, Black, AP
Instrument Part 9240678 Bimba Rotary Actuator, AP
Instrument Part 9018129 Belt, x, extractor, Qsym
Instrument Part 9018150 PCB, interface, drawer, Qsym
Instrument Part 9240949 Cable, y, portal, Qsym
Instrument Part 9240960 Cable, cLLD, pipetting system, Qsym
Consumable 1062791 HBV PCR REAGENT III
Instrument Part 9243045 Bracket, rear, transparent panel, Qsym
Consumable 292143 mericon Horse Kit (24)
Instrument Part 9019074 PyroMark Storage Box 13x8x5cm, Pyro Box for dust free and safe storage of tips in-between runs in PyroMark Q96 MD.
Consumable 9019077 PyroMark Assay Design SW 2.0 All-in-one software for design of both PCR and sequencing primers
Consumable 979012 Fungi ITS2 Primers For PCR
Consumable 979301 PyroMark Q24 Plate (100) CE 24 well plate for samples, for use in PyroMark Q24. 100 plates in each package. CE-marked
Accessory 19505 QIAvac HiSpeed LS
Consumable 990602 Elution Plate (10)
Consumable 206672 Type-it CNV SYBR Green PCR +qC Kit (100)
Consumable 10083 QIAGEN-tip 2500 (25) 25 columns; 2.5 mg column capacity
Consumable 12143 QIAGEN Plasmid Midi Kit (25) 25 QIAGEN-tip 100, Reagents, Buffers
Consumable 19052 Buffer P2 500 ml Lysis Buffer
Consumable 12245 QIAfilter Plasmid Midi Kit (100) 100 QIAGEN-tip 100, Reagents, Buffers, 100 QIAfilter Midi Cartridges
Consumable 27106 QIAprep Spin Miniprep Kit (250) For 250 high-purity plasmid minipreps: 250 QIAprep Spin Columns, Reagents, Buffers, Collection Tubes (2 ml)
Accessory 81031 Plate Rotor 2 x 96 Rotor for 2 QIAGEN 96-well plates, for use with QIAGEN Centrifuges
Consumable 19065 Buffer PE (concentrate) 100 ml Wash Buffer (5x concentrate for 500 ml buffer)
Consumable 19082 Buffer ASL (4 x 140 ml) 560 ml Stool Lysis Buffer
Consumable 20021 QIAEX II Gel Extraction Kit (150) For 150 extractions: 3 x 0.5 ml QIAEX II Suspension, Buffers