詳細介紹
高遷移率族蛋白B1(HMGB-1)檢測試劑盒Human High mobility group protein B1,HMGB-1 ELISA Kit高品質elisa試劑盒,齊一生物科技有限公司提供的試劑盒受到了廣大科研單位的*肯定和認同.*保證,價格公道,傾力為國內外科研院校實驗室提供優質的產品.若有需要,我司將竭誠為您服務
高遷移率族蛋白B1(HMGB-1)檢測試劑盒有效期:6個月
預期應用:ELISA法定量測定血清、血漿、細胞培養上清或其它相關生物液體中的含量.
說明
1.試劑盒保存:-20℃(較長時間不用時);2-8℃(頻繁使用時).
2.濃洗滌液低溫保存會有鹽析出,稀釋時可在水浴中加溫助溶.
3.中、英文說明書可能會有不*之處,請以英文說明書為準.
4.剛開啟的酶聯板孔中可能會含有少許水樣物質,此為正常現象,不會對實驗結果造成任何影響.
實驗原理
用純化的抗體包被微孔板,制成固相載體,往包被抗該指標抗體的微孔中依次加入標本或標準品、*化的抗該指標抗體、HRP標記的親和素,經過*洗滌后用底物TMB顯色.TMB在過氧化物酶的催化下轉化成藍色,并在酸的作用下轉化成zui終的黃色.顏色的深淺和樣品中的該指標呈正相關.用酶標儀在450nm波長下測定吸光度(OD值),計算樣品濃度.
試劑盒組成及試劑配制
1. 酶聯板(Assay plate ):一塊(96孔).
2. 標準品(Standard):2瓶(凍干品).
3. 樣品稀釋液(Sample Diluent):1×20ml/瓶.
4. *標記抗體稀釋液(Biotin-antibody Diluent):1×10ml/瓶.
5. 辣根過氧化物酶標記親和素稀釋液 (HRP-avidin Diluent):1×10ml/瓶.
6. *標記抗體(Biotin-antibody):1×120μl/瓶(1:100)
7. 辣根過氧化物酶標記親和素(HRP-avidin):1×120μl/瓶(1:100)
8. 底物溶液(TMB Substrate):1×10ml/瓶.
9. 濃洗滌液(Wash Buffer):1×20ml/瓶,使用時每瓶用蒸餾水稀釋25倍.
10. 終止液(Stop Solution):1×10ml/瓶(2N H2SO4).
10、標本的采集及保存
1. 血清:全血標本請于室溫放置2小時或4℃隔夜后于1000 x g離心20分鐘,取上清即可檢測,或將標本放于-20℃或-80℃保存,但應避免反復凍融.
2. 血漿:可用EDTA或肝素作為抗凝劑,標本采集后30分鐘內于2 - 8° C 1000 x g離心15分鐘,或將標本放于-20℃或-80℃保存,但應避免反復凍融.
3. 細胞培養物上清或其它生物標本:1000 x g離心20分鐘,取上清即可檢測,或將標本放于-20℃或-80℃保存,但應避免反復凍融.
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Consumable 12263 QIAfilter Plasmid Maxi Kit (25) 25 QIAGEN-tip 500, Reagents, Buffers, 25 QIAfilter Maxi Cartridges
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Accessory 19155 QIAGEN Protease (7.5 AU) 7.5 Anson units per vial (lyophilized)
Consumable 27193 QIAprep 96 Turbo Miniprep Kit (24) For 24 x 96 high-purity plasmid minipreps, 24 each: TurboFilter 96 and QIAprep 96 Plates; Flat-Bottom Blocks and Lids, Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
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Instrument Part 9239089 User Manual, BioSprint 15
Instrument Part 9240539 Cable Assy, Home Sensor, SiNug, AP96/196
Instrument Part 9240584 Filter Assembly, Vacuum, AP196/98
Instrument Part 9240763 Analog Module, F2-04AD-1, AP96
Instrument Part 9240669 Male Quick Disconnect, AP
Instrument Part 9240689 Jam nut, Shaker Bimba, AP98
Instrument Part 9018159 Touch screen, Qsym
Instrument Part 9018131 Drop catcher, extractor, Qsym
Instrument Part 9018175 Optical sensor, Qsym
Instrument Part 9240944 Motor, xy, portal, Qsym
Instrument Part 9240974 Carrier locking, sample tracking, Qsym
Instrument Part 9240963 Drop catcher, pipetting system, Qsym
Instrument Part 9240981 Slide block, sample tracking, Qsym
Instrument Part 9240986 PCB, main, cooling device, Qsym
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9021694 Washer Boot HPV Media
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Instrument Part 9238676 Lid, speed measurement
Instrument Part 9238695 Voltage regulator 7805 DC, 5 V
Instrument Part 9238767 Swing-out rotor for microplate, sealable
Instrument Part 9238787 Angle rotor 30 x 1.5 / 2.2 ml
Instrument Part 9238807 Round carrier for 12 Tubes 15ml
Instrument Part 9238822 Rect. carrier for 1 tube 100 ml
Instrument Part 9235702 Cables, Shaker, RCS1
Instrument Part 9017344 CD User Manual LiquiChip 200
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Instrument Part 9016874 Kit, Maint. Prot. Plasticware, BR UNIV
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Consumable 301802 HiPerFect HTS Reagent (0.1 ml)
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Instrument Part 9238607 Teflon disc, motor bearing protection
Instrument Part 9238621 Power supply, motorized lid locks
Instrument Part 9238646 Diode BY 500-600, 600 V 5 A
Instrument Part 9238667 Front panel
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